Cell biology lab western blot results12/27/2023 ![]() “As far as lot-to-lot variability is concerned, we’re testing every lot that we bring in,” says Mark Shulewitz, a senior scientist in the Content Business Development Team at Bio-Rad. The new line is a targeted approach to overcoming many of the aforementioned industry challenges.īy subscribing, you agree to receive email related to Lab Manager content and products. Bio-Rad, for example, is pioneering this approach with the introduction of its PrecisionAb™ Antibody product line, specifically validated for western blots. An alternative approach to ensure reliability is for vendors to narrow antibody selections. Validating for a broad range of antibody applications would be prohibitively expensive, driving up product prices. Solution: Vendors cannot realistically validate antibodies for every possible application and sample treatment or type. Furthermore, manufacturing and quality control standards can be lax, which means there is no guarantee that buying different lots of the same antibody from the same supplier will produce the same results. One reason this occurs is because vendors provide insufficient validation information, leaving researchers in the dark about the antibody’s true binding efficiency and overall performance. ![]() Scientists using non-specific antibodies have gone on to unknowingly publish erroneous data. Even if the antibody does work, it may bind to other proteins in addition to the target protein or may not be sensitive enough to detect low abundance targets, complicating analysis of the results. B) This cyclin-dependant kinase 2 (CDK2) mouse monoclonal antibody passed validation showing high specificity and sensitivityĪ recent Bio-Rad survey found nearly 60 percent of researchers blamed the primary antibody when their immunoblots failed. A) This carbonic anhydrase IX (CA9) mouse monoclonal antibody failed validation because it exhibits nonspecific binding and low signal-to-noise ratio. Validation data for two PrecisionAb candidates.
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